Click-it edu kit
WebFeb 15, 2009 · An alternative thymidine analogue is 5-ethynyl-2′-deoxyuridine (EdU), in which the terminal methyl group is replaced with an alkyne group, which allows detection using a fluorescent azide that covalently binds to the alkyne group in a reaction known as “click chemistry” (Hsu et al., 2007, Sawa et al., 2006, Tornoe et al., 2002) (Fig. 1).This … WebMake 10X Click-iT® EdU buffer additive by adding 2 mL deionized water to Component F and mixing. Store at –20°C; Label cells with EdU. Plate cells on coverslips and incubate …
Click-it edu kit
Did you know?
WebMar 9, 2015 · the Click-iT® EdU assay uses bioorthogonal (biologically unique) moieties, producing low backgrounds and high detection sensitivities. The kit contains all of the …
WebClick-iT® Plus EdU Flow Cytometry Assay Kits, 100 tests Catalog numbers C10633, C10635 Table 1 Contents and storage Material Amount Concentration Storage* Stability … Webwahoo.nsm.umass.edu
WebThe Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in … WebMar 9, 2015 · Click-iT® EdU Flow Cytometry Assay Kits *50 tests* 5 Labeling Cells with EdU 2.1uspend the cells in an appropriate tissue culture medium to obtain optimal …
WebThe Click-iT EdU Alexa Fluor 488 Imaging Kit is a superior alternative to traditional proliferation assays that is optimized for fluorescence microscopy applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor dye in a ...
WebAug 12, 2024 · To make a 10X stock solution of the Click-iT® EdU buffer additive (Component F): Add 2 mL deionized water to the vial, then mix until fully dissolved. After … open houses in sylmar caWebEdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. With Click-iT® EdU, mild fixation and detergent permeabilization is sufficient for the small molecule-based Click-iT® EdU detection reagent to gain access to the DNA. As a consequence, the Click-iT® EdU HCS assay is ... open houses in the villages tomorrowWeb2 days ago · Meijer is voluntarily recalling salad kits under its brand name due to possible listeria contamination. The FDA announced the recall on April 10 that there is a voluntary … open houses in the villages flWebThe Click-iT EdU Alexa Fluor 555 Imaging Kit provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated … open houses in the villages fl tomorrowWebClick-iT Plus EdU 细胞增殖成像试剂盒已优化并应用于荧光显微镜，且这是一种优于传统增殖检测的方法。. 本测定试剂盒中经修饰的胸腺嘧啶核苷类似物 EdU（5-乙炔基-2'-脱氧尿苷，一种胸腺嘧啶核苷的核苷类似物）能有效的掺入新合成的 DNA 并且通过明亮的、非 ... open houses in toledo ohio 43606Webmethod. In contrast, the Click-iT® Plus EdU cell proliferation kit is compatible with cell cycle dyes, R-PE, R-PE tandems, and fluorescent proteins such as GFP, RFP, and mCherry. Figure 2 below demonstrates Click-iT® Plus EdU multiplexed with Hu CD3 PE and Figure 3 (page 3) shows Click-iT® Plus EdU multiplexed with FxCycle™ violet and GFP. open houses in taunton maWebClick-iT EdU 技术的优势在于化学方面 - 独特的小分子和低背景的标记物以及检测基团之间特异性共价反应。 5-乙炔基-2'-脱氧尿苷（EdU）是含有炔烃的核苷类似物。 在铜催化 … open houses in upper marlboro