Phospho explorer antibody array
WebMar 18, 2024 · The Phospho Explorer Antibody Array kit used in this study was obtained from Full Moon BioSystems (catalog PEX100; Sunnyvale, CA, USA) and covered 1318 well-characterized antibodies, the phosphorylated and non-phosphorylated state of these proteins will be detected at the same time (Pulito et al. 2016 ). WebMar 25, 2016 · Phospho-protein profiling by Phospho Explorer Antibody Array Cell lysates obtained from PC-1 and PC-1.0 cells were applied to a Phospho Explorer Antibody Array (Full Moon Biosystems, Sunnyvale, CA, USA). The array contained 1318 antibodies.
Phospho explorer antibody array
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WebMay 13, 2024 · Phosphodiesterase type 5 (PDE5) inhibitors are the most commonly used first-line treatment options for ED; however, these agents are ineffective in ~30% of patients and ultimately cannot rescue... Web46 rows · The Proteome Profiler Human Phospho-Kinase Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Phosphorylation of captured proteins is detected with biotinylated phospho-specific detection antibodies …
WebImmunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190). Immunofluorescence; Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190, red). WebWe used the Phospho Explorer Antibody Array, which encompasses nearly all known signaling pathways, to assess molecular mechanisms underlying LPHN2-induced cardiac differentiation. LPHN2-dependent phosphorylation was the strongest for cyclin-dependent kinase 5 (CDK5) at Tyr15. We identified CDK5, Src, and P38MAPK as key downstream …
WebPhospho Explorer Array is a glass-based Array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples. It is a high-throughput ELISA based antibody microarray for qualitative/semi-quantitative protein … WebMar 25, 2016 · Phospho-protein profiling by Phospho Explorer Antibody Array. Cell lysates obtained from PC-1 and PC-1.0 cells were applied to a Phospho Explorer Antibody Array (Full Moon Biosystems, Sunnyvale, CA, USA). The array contained 1318 antibodies. Each of the antibodies has two replicates that are printed on coated glass microscope slide, along …
WebPhospho Explorer Antibody Array data of KGN cells treated with Hu‐17. (A) Heatmap of proteins differently phosphorylated proteins in KGN cells with Hu‐17 treatment. The data included in the heatmap is beyond cutoff line with the change fold is ≤77% or ≥130%. A is the control group, B is the Hu‐17 treated for 3 h group, C is the Hu ...
WebOne of the best selling antibody arrays with 269 key phospho-specific and site-specific antibodies from multiple cancer signaling pathways. Cytokine Profiling Array Featuring 310 cytokine related antibodies. Each antibody is printed with six replicates. Ideal for cytokine profiling and inflammation studies. Phospho Explorer Array irish stoolsWebSep 1, 2024 · Total RNA was extracted by chloroform and isopropanol precipitation. cDNA was obtained using the SuperScript III First-Strand Synthesis System (Invitrogen, 18080-051). qPCR analyses were performed with primers listed below using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725270) in at least duplicate in three … irish stone blessing for weddingWebProteome Profiler Antibody Arrays are simple and economical multiplex assays for early-stage discovery research. This semiquantitative antibody array allows for the measurement of more than 100 proteins in a single … port everglades forecast by tideworksWebarray antibody pairs are optimised to provide the most accurate and reliable data. Human Cytokine Array Panel A (Cat# ARY005B) PBMCs untreated (top) or treated with ... p27/Kip1, PON2, Phospho-p53 (S15), Phospho-p53 (S46), Phospho-p53 (S392), Phospho-Rad17 (S635), SMAC/Diablo, Survivin, TNF RI/TNFRSF1A, TRAIL R1/DR4, TRAIL R2/DR5, XIAP ... port everglades fit terminalWebMay 6, 2014 · Several phosphokinase antibody arrays have been developed , including Phospho Explorer Array (Full Moon BioSystems), Signal Transduction AntibodyArray (Hypromatrix), Kinex Antibody Microarray (Kinexus Bioinformatics), Human Phospho-Kinase Antibody Array (R&D system), and Panorama Antibody Array XP725 (Sigma-Aldrich). port everglades cruise parking feesWebOct 27, 2024 · Phospho Explorer antibody array experiments and analyses were performed as a custom service by E-Biogen (Ebiogen Inc., Seoul, Republic of Korea). Overall design HUVECs were serum-starved for 8 hours and then incubated with an anti-TGFβ1 antibody for 1 hour, after which HUVECs were stimulated with 1μg/ml DG-LRG1 for 30 minutes. irish store annapolis mdWebCompared to the conventional methods, our antibody arrays provide a much more cost-effective means for protein research. Investigators can choose from over 40 different arrays to quickly compare protein expression or … port everglades cruise parking rates